Rough, opaque, circular colonies that turned blue when sporulation
occurred. Emitted an odor that was very characteristic of soil.
Positive Result
Motility Stab
Single stab line apparent through the length of the agar
in tube.
Gram Stain
Long filamentous strands can be seen as they branch out
from a mass of Streptomyces cells. The cells stained purple which
reveals and also confirms that our organism is gram positive. Streptomyces
are characteristically gram positive.
Negative
/Positive Result
MacConkey's Plates
Growth was not seen on MacConkey's Plates. Confirmed the
Gram Stain result. MacConkey's agar prohibits the growth of gram positive
organisms.
Sugar Fermentation Tubes
Color change in second tube without oil indicates oxidative
metabolism which is characteristic for aerobes. Color change in third tube
with oil indicates fermentative metabolism. Color change in both tubes
suggest a facultative anaerobe.
Positive Result
Catalase Test
Bubbles formed on addition of the cells to hydrogen peroxide.
click on image to enlarge.
Antibiotic Test Click on image above to enlarge
Plates: Upper Left: E.Coli - Upper Right: S. Marcenes Lower
Right: B. Subtillus Lower
Left: S. Aureus