Materials:

9 clean, dry petri dishes
9 soil samples
40 plates per media; 3 media used (GYM media, selective streptomyces agar, and starch caesin)
10 motility slants
8 sugar fermentation tubes
Mineral oil
glass slides
H₂O₂
MacConkey's plates
Four organisms: Serratia marcenes, Staphylococcus aureus, Bacillus subtillus, and Escherichia coli
Scotch tape (required for tape mount)
Anaerobic chamber
 

1.) Place about 15 grams of each soil sample into separate empty petri dish.  Dry (heat) for at least 1.5 hours.

2.) Using a spatula or scoopula, transfer 0.5 inches of sample into test tube with 100mL of saline solution.  Vortex.

3.)  Immediately transfer plate 100uL of mixture onto plate.  Spread evenly.  Cover and incubate one plate per temperature (23°C and 29°C).  Observe after 5-7 days.

4.) Choose colonies sharing physical characteristics of streptomyces.  Restreak each colony on starch casein media until pure colonies are obtained.

5.) Test each pure colony in following eight (8) tests:

a.) Gram stain
b.) MacConkey’s (confirm gram stain)
c.) Motility stabs
d.) Catalase test
e.) Tape mount
f.) Anaerobic culture jar growth
g.) Sugar fermentation tubes
h.) Antibiotic test