The purpose of this study was to enrich for Acetobacter from several environmental samples where Acetobacter is commonly found. We hypothesized that Acetobacter could be enriched for by following our protocol, which included selective growth conditions as well as several standard microbial tests that aided in identification and characterization of Acetobacter. The selective medium used was Hoyer’s Media, while YGC medium was used for culture maintenance. Tests used for identification and characterization included light microscopy (morphology), Gram stain, endospore stain, oxidase test, catalase test, motility stab, and carbohydrate fermentation tests. Two microorganisms were isolated. Both organisms were catalase positive, oxidase negative, motile, ethanol fermenting, obligatory aerobic bacilli. One organism was Gram variable while the other was Gram negative. Based on the results, we concluded that characteristics of the two microorganism isolated were in agreement with those characteristics of Acetobacter outlined in the literature. Moreover, our protocol was successful in enriching for Acetobacter.