1)  Prepare Pseudomonas broth and autoclave.
2)  Collect 2 inch deep soil samples using a soil core.

Day 2:

1)  Prepare Pseudomonas Isolation Agar and autoclave.
2)  Pour 10ml broth into 20ml test tubes using 2 test tubes for each sample.
3)  Inoculate each test tube with 0.5g of soil.
4)  Incubate tubes at 27 ^oC for 48 hours in a shaker incubator.

Day 3:

1)  Steak Pseudomonas Isolation Agar plates with Pseudomonas broth, 2 using 2 plates per test tube.
2)  Incubate plates at 27 ^oC for 72 hours in the dark.
3)  Prepare Pseudomonas Oil Agar and autoclave.

Day 4:

1)  Streak isolated colonies from Pseudomonas Isolation Agar onto the Pseudomonas Oil plates.
2)  Incubate plates at 27oC for 92 hours in the dark.