Restriction Enzymes

Biochemical Engineering

A List of Restriction Enzymes from Sigma

Recognition   Enzymes
Sites
---------------------------------------
4 Bases
---------------------------------------
cg/cg    AccII, BstU I, FnuD II, Tha I
ag/ct    Alu I
gcg/c    Cfo I, Hha I
ga/tc    Dpn I
gg/cc    Hae III,  Pal I
g/cgc    HinP I
c/cgg    Hpa II, Msp I
c/tag    Mae I
a/cgt    Mae II
/gatc    Mbo I, Nde II, Sau3A I
t/taa    Mse I
gt/ac    Rsa I
t/cga    Taq I, TthHB8 I
---------------------------------------
5 Bases
---------------------------------------
cc/agg   Apy I*, BstN I*
cc/tgg   Apy I*, BstN I*
g/gacc   Asu I*, Nsp IV*, Sau96 I*
g/gtcc   Asu I*, Nsp IV*, Sau96 I*
g/gccc   Asu I*, Nsp IV*, Sau96 I*
g/ggcc   Asu I*, Nsp IV*, Sau96 I*
g/gacc   Ava II*, Sin I*
g/gtcc   Ava II*, Sin I*
c/taag   Dde I*
c/ttag   Dde I*
c/tcag   Dde I*
c/tgag   Dde I*
/ccagg   EcoR II*
/cctgg   EcoR II*
gc/agc   Fnu4H I*
gc/tgc   Fnu4H I*
gc/cgc   Fnu4H I*
gc/ggc   Fnu4H I*
g/aatc   Hinf I*
g/attc   Hinf I*
g/actc   Hinf I*
g/agtc   Hinf I*
/gtaac   Mae III*
/gttac   Mae III*
/gtcac   Mae III*
/gtgac   Mae III*
cc/cgg   Nci I*
cc/ggg   Nci I*
---------------------------------------
6 Bases
---------------------------------------
gacgt/c  Aat II
gt/atac  Acc I*
gt/cgac  Acc I*
c/ttaag  Afl II
ga/cgtc  Aha II*
gg/cgcc  Aha II*
gggcc/c  Apa I
g/tgcac  ApaL I
at/taat  Ase I
g/gtacc  Asp718 I
c/ccggg  Ava I*
c/tcgag  Ava I*
c/ctagg  Avr II
tt/cgaa  Asu II, BstB I
tgg/cca  Bal I
g/gatcc  BamH I
g/gcgcc  Ban I*
g/gtacc  Ban I*
gagct/c  Ban II*
gagcc/c  Ban II*
gggct/c  Ban II*
gggcc/c  Ban II*
ggcgc/g  Bbe I
t/gatca  Bcl I
a/gatct  Bgl II
ga/cgtc  BsaH I*
gg/cgcc  BsaH I*
gagct/c  Bsp1286 I*
gggcc/c  Bsp1286 I*
gtgca/c  Bsp1286 I*
t/ccgga  BspE I
t/catga  BspH I
t/ccgga  BspM II
g/cgcgc  BssH II
g/gatcc  Bst I
at/cgat  Cla I
ttt/aaa  Dra I
c/ggccg  Eae I*
t/ggcca  Eae I*
c/ggccg  Eag I
g/aattc  EcoR I
gat/atc  EcoR V
tgc/gca  Fsp I
agcgc/t  Hae II*
ggcgc/c  Hae II*
gagct/c  HgiA I*
gtgca/c  HgiA I*
ga/cgtc  HgiD I*
gg/cgcc  HgiD I*
gtc/gac  Hine II*
gtt/aac  Hine II*
gtc/gac  Hind II*
gtt/aac  Hind II*
a/agctt  Hind III
gtt/aac  Hpa I
ggtac/c  Kpn I
a/cgcgt  Mlu I
tgg/cca  Msc I
gcc/ggc  Nae I
gg/cgcc  Nar I
c/catgg  Nco I
ca/tatg  Nde I
g/ctagc  Nhe I
gga/tcc  Nla IV*
ggt/acc  Nla IV*
ggc/gcc  Nla IV*
ggg/ccc  Nla IV*
tcg/cga  Nru I
atgca/t  Nsi I
gagct/c  Nsp II*
gggcc/c  Nsp II*
gtgca/c  Nsp II*
c/ccggg  Nsp III*
c/tcgag  Nsp III*
c/tcgag  RaeR7 I
ctgca/g  Pst I
cgat/cg  Pvu I
cag/ctg  Pru II
cgat/cg  Rsp Ia
gagct/c  SacI
ccgc/gg  Sac II
g/tcgac  Sal I
agt/act  Sca I
ccc/ggg  Sma I
tac/gta  SnaB I
a/ctagt  Spe I
gcatg/c  Sph I
aat/att  Ssp I
gagct/c  Sst I
ccgc/gg  Sst II
agg/cct  Stu I
c/catgg  Sty I*
c/ctagg  Sty I*
t/ctaga  Xba I
c/ccggg  Xcy I
c/tcgag  Xho I
a/gatct  Xho II*
g/gatcc  Xho II*
cgat/cg  Xor II
c/ccggg  Xma I
c/ggccg  Xma III

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Biochemical Engineering -- Restriction Enzymes
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Nam Sun Wang

Department of Chemical & Biomolecular Engineering

University of Maryland

College Park, MD 20742-2111

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