Biochemical Engineering CHBE482

CHBE482

Biochemical Engineering

Spring 2016

Class Handouts & Lecture Notes

Class Syllabus
Policy on Academic Integrity
Homework
Week 1, 01/26 Snow
Week 1, 01/28 Syllabus, Terminology, Chemical Engineering vs. Biochemical Engineering
Week 2, 02/02 The Rise of a Wonder Drug, Brief History, Applications
Week 2, 02/04 NCBI (National Center for Biotechnology Information), Taxanomy, Nomenclature, Classification, Kingdom of Protists, Prokaryotes/Eukaryotes
Week 3, 02/09 Virus, Bacteria, Yeasts, Biochemistry (Carbohydrates, lipids, amini acid, protein
List of Amino Acids
Zwitterion Behavior of Amino Acid
Demo (Density Gradient Centrifugation)
Week 3, 02/11 Biochemistry (RNA/DNA)
Week 4, 02/16 Catalysis, Michaelis-Menten kinetics
Week 4, 02/18 Michaelis-Menten kinetics, ODE solving
Week 5, 02/23 Enzyme inhibition, pH & temperature effect
Week 5, 02/25 Enzyme immobilization (external mass transfer)
Demo (catalyst supports)
Week 6, 03/01 Enzyme immobilization (internal mass transfer)
Demo (gel entrapment)
Week 6, 03/03 Mid-term exam #1
Week 7, 03/08 Review mid-term exam #1, enzyme immobilization
Week 7, 03/10 Central Dogma of Biology: replication, DNA sequencing, PCR, artificial sequence & insulin
Week 8, 03/22 Central Dogma of Biology: transcription, promoter/operator, induction/repression, translation, codon translation table
Week 8, 03/24 Metabolic Pathways, oxidation/reduction, NAHD, ATP, glycolysis, & TCA, energy efficiency
Week 9, 03/29 Genetic Engineering, restriction enzyme, ligase, PCR, primers
Week 9, 03/31 transformation, transfection
Week 10, 04/05 Stoichiometry
Week 10, 04/07 Stoichiometry, batch cell growth
Week 11, 04/12 Cell growth kinetics, structured model
Week 11, 04/14 Mid-term exam #2
Week 12, 04/19 CSTR, fed-batch
Week 12, 04/21
Week 13, 04/26 Plasmid instability, two-compartmental model for incomplete mixing, cell recycle
Week 13, 04/28 oxygen mass transfer, sterilization
Week 14, 05/03 purification
Demo (Density Gradient Centrifugation)
Week 14, 05/05 purification
Week 15, 05/10 cell culture
Week 16, 05/17 1:30pm-3:30pm Final exam

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Biochemical Engineering
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Nam Sun Wang

Department of Chemical & Biomolecular Engineering

University of Maryland

College Park, MD 20742-2111

301-405-1910 (voice)

301-314-9126 (FAX)