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@T$I$@%4-k@{;% ?$I$@%,T?%$I$@%n?%$I$@%u^:%$I$@%1?%$I$@%&3D?%$I$@% 4]? @U%I$I@% &:o;% ?%I$I@%*-?%%I$I@%F(?%%I$I@%. 4KZ:%%I$I@%~?%%I$I@%ZBD?%%I$I@% :? @V@@%  C;% ?@@%Dlɢ?%@@%\?#?%@@%4K:%@@%j.?%@@%UD?%@@% XWX? @W۶m۶m@%Ni~y]:% ?۶m۶m@%!3"L?%۶m۶m@%?%۶m۶m@%W`;%۶m۶m@%x:ѐ?%۶m۶m@%4D?%۶m۶m@% "w5Y? @Xm۶m@%=ZW~:% ?m۶m@%y$Щn>%m۶m@%bkE?%m۶m@%&%<%m۶m@%T?%m۶m@%0}D?%m۶m@% pg? @Y$I$ɀ@%G &I:% ?$I$ɀ@%>%$I$ɀ@%O=?%$I$ɀ@%Ҝ<%$I$ɀ@%fcP ?%$I$ɀ@% |D?%$I$ɀ@% 052y? @Zn۶m@%:[ :% ?n۶m@%*`0>%n۶m@%f?%n۶m@%ou0g=%n۶m@%,0?%n۶m@%|D?%n۶m@% u ? @[I$I$@%T9% ?I$I$@%=%I$I$@%i1?%I$I$@%F h=%I$I$@%0B?%I$I$@%|D?%I$I$@% K.? @\%I$IR@%LN9% ?%I$IR@%q2zv>%%I$IR@%?%%I$IR@%& Wyv>%%I$IR@%I*Mȵ?%%I$IR@%|D?%%I$IR@% ? @]@%JGyTd9% ?@% >%@%C>?%@%X >%@%Sɢ?%@%|D?%@% ʈ(? @^ܶm۶@%[|+9% ?ܶm۶@% V]I?%ܶm۶@%{f ?%ܶm۶@% V]I?%ܶm۶@%: ?%ܶm۶@%|D?%ܶm۶@% ʑ? @_m۶mہ@%hvB8% ?m۶mہ@%$H-?%m۶mہ@%,?%$I$ @%|D?%$I$ @% ƣ? @an۶m6@%nhw8% ?n۶m6@%Y,@%n۶m6@%k͸@%n۶m6@%Y,@%n۶m6@%w@%n۶m6@%|D?%n۶m6@% dDbl? @bI$Id@%AvE98% ?I$Id@% 10 recalc range O8..S17 recalc range M10 put X1 into X2 put O12 into X1 put (X1+X2)/2 into X1 recalc range M12 put 0 into W1 end if put W1+1 into W1 end if end idle Pp~ d dG9x; Ni@@$@$@F$@$@$@ <22@2(  dd ~  d  dGqx; i@@$@$@F$@$@$@ <22@2(  d  d ~{ddGMox; i@@$@$@F$@$@$@ <22@2(dd numberwheel2/wp !% numberwheel2!U@.5                put ctvalue("numberwheel2",0) into U6 put indirect("U"&6+U6) into K12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 System System@?4@? numberwheel.wp   $ numberwheel!U@.5                put ctvalue("numberwheel",0) into U6 put indirect("U"&6+U6) into I12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 System System @?4@?Remove cuvette'Remove8    ."Cuvette removed: Insert to measure               put 0 into X1 put 0 into Z1 put 0 into Q14 put "Cuvette removed: Insert to measure" into I16 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M120 Chicago ChicagoRInsert cuvette&ϘInsert/  h㈵> Cuvette in place               select range I12 put 1 into Z1 put 0.00001 into Q14 put "Cuvette in place" into I16 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M120 Chicago ChicagoI$No}   Chicago Geneva@,Offset Geneva(( VertSlider#No}  $%%%%# VertSlider put ctvalue("VertSlider",0) into M8 Chicago Geneva987@Y@?Y@ "p"l  Chicago Geneva@,Emission wavelength Geneva((!j Chicago Geneva@,Excitation wavelength Geneva((EmissionSlider { p^^^^'EmissionSlider      put ctvalue("EmissionSlider",0) into k8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 Chicago Genevaǿ"|@i@@?y@ExcitationSlidepr````)ExcitationSlider     put ctvalue("ExcitationSlider",0) into I8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 Chicago Geneva"BXx@i@@?y@Scan both monocP Scan both monochromators  i@@@@@.'   61g@?`n@  61g@?`n@  61@U@2@Z@o@       countselect range I12 manual recalc define count put I8 into V1 put k8 into V2 for count = 200 to 600 step 2 put count into I8 put count into k8 end for repaint off select object 9 Object Location frac(A128..G152,191,0,1,243) select object 10 Object Location frac(A128..G152,191,0,1,243) recalc select object 11 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect put V1 into I8 put V2 into k8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M120 Chicago ChicagoS+ Scan Emission   Scan Emission  i@@?@?.   61g@?`n@  61g@?`n@  61@U@2@Z@o@       countselect range I12 manual recalc define count put k8 into V2 for count = 200 to 600 put count into k8 end for repaint off select object 9 Object Location frac(A128..G152,191,0,1,243) select object 11 Object Location frac(A128..G152,191,0,1,243) recalc select object 10 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect put V2 into k8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M120 Chicago ChicagoS.Scan Excitation  Scan Excitation i@@?@?.  61g@?`n@  61g@?`n@  61@U@2@Z@o@      countselect range I12 manual recalc define count put I8 into V1 for count = 200 to 600 put count into I8 end for repaint off select object 11 Object Location frac(A128..G152,191,0,1,243) select object 10 Object Location frac(A128..G152,191,0,1,243) recalc select object 9 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect put V1 into I8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M120 Chicago ChicagoS$30000utton 30 ZZZZ         select chart 9 axis 3 manual scaling from 0 to 30 with 3 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 30 with 3 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 30 with 3 major and 5 minor divisions unselect% Chicago Geneva3,30000uttonղ 3000ZZZZ            select chart 9 axis 3 manual scaling from 0 to 3000 with 3 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 3000 with 3 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 3000 with 3 major and 5 minor divisions unselect% Chicago Geneva3(30000utton 300 ZZZZ   ,   ,   ,select chart 9 axis 3 manual scaling from 0 to 300 with 3 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 300 with 3 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 300 with 3 major and 5 minor divisions unselect% Chicago Geneva3`P1  Chicago GenevammA`, Fluorescence Spectrophotometry Simulation of a two-component mixture. To change the concentrations of the components A and B, click on the up and down arrows to the left of the concentration displays. (Concentration range is zero to 100 ppm in a 1,2, 5, 10 sequence). Or you can type in any concentration while the cuvette is removed. To change the excitation and emission wavlengths (in nm), adjust the two sliders at the bottom. Fluorescence intensity (in arbitrary units) and the absorbance of the solution at the excitation wavelength are displayed in the black boxes. Readings are continuous as long as the cuvette is inserted into the instrument. (The random variations in readings are due to photon noise). To scan a spectrum, click on the corresponding scan button. Change the y-axis scale of the plots by clicking on one of the seven sensitivity buttons below. To obtain a synchronous spectrum, set the wavelength offset with the slider on the right and click "Scan both monochromators". Note: the intensity and absorbance displays respond immediately to changes in concentrations and wavelengths; however, spectra must be re-scanned after changes. Geneva Geneva Geneva Geneva((./Auto0uttonЬ AutoHHHH      select chart 9 axis 3 automatic scaling select chart 10 axis 3 automatic scaling select chart 11 axis 3 automatic scaling unselect % Chicago GenevaA,1000Buttonղ 1000ZZZZ         select chart 9 axis 3 manual scaling from 0 to 1000 with 5 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 1000 with 5 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 1000 with 5 major and 5 minor divisions unselect% Chicago Geneva1(100 Button 100 ZZZZ   d   d   dselect chart 9 axis 3 manual scaling from 0 to 100 with 5 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 100 with 5 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 100 with 5 major and 5 minor divisions unselect% Chicago Geneva1)10w Button * 10ZZZZ             select chart 9 axis 3 manual scaling from 0 to 10 with 5 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 10 with 5 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 10 with 5 major and 5 minor divisions unselect % Chicago Geneva1@+  @)0^ |88,,x,, ~ y be(,, d'KB@ Q54