User-suplied inputs: Column B Concentrations of the standards (X) Column C Instrument readings of the standards (Y) Column J Instrument Readings of the unknowns Calculated outputs: Cell B28 n = number of x,y data points Cell B27 sumx = ?x Cell C27 sumy = ?y Cell D27 sumxy = ?x*y Cell E27 sumx2 = ?x*x Cell F27 sumx3 = ?x^3 Cell G27 sumx3 = ?x^4 Cell H27 sumx2y = ?(x^2)*y Cell B30 D = n*sumx2*sumx4+2*sumx*sumx2*sumx3-sumx2^3-sumx^2*sumx4-n*sumx3^2 Cell B31 a = (n*sumx2*sumx2y+sumx*sumx3*sumy+sumx*sumx2*sumxy-sumx2^2*sumy-sumx^2*sumx2y-n*sumx3*sumxy)/D Cell B32 b = (n*sumx4*sumxy+sumx*sumx2*sumx2y+sumx2*sumx3*sumy-sumx2^2*sumxy-sumx*sumx4*sumy-n*sumx3*sumx2y)/D Cell B33 c = (sumx2*sumx4*sumy+sumx2*sumx3*sumxy+sumx*sumx3*sumx2y-sumx2^2*sumx2y-sumx*sumx4*sumxy-sumx3^2*sumy)/D Column K Calculated concentration = (-b+SQRT(b*b-4*a*(c-InstrumentReading)))/(2*a)