|
Current Homework
|
Today's Class Handouts
|
Nam Sun Wang
's ...
ENCH482
Biochemical Engineering
Fall 2010
Homework
Class Syllabus
Policy on Academic Integrity
Review of Lectures
Review of Computational Methods
8/30 Syllabus, Terminology, Chemical Engineering vs. Biochemical Engineering
9/01 Brief History, Applications, Nomenclature, Classification, Kingdom of Protists, Virus, Bacteria, Yeasts, Prokaryotes/Eukaryotes
NCBI (National Center for Biotechnology Information)
, Taxanomy
9/03 Biochemistry (Carbohydrates, Lipids)
9/06 Holiday (Labor Day)
9/08 Biochemistry (Amino Acid, Protein)
List of Amino Acids
Zwitterion Behavior of Amino Acid
Demo (Density Gradient Centrifugation)
9/08 Biochemistry (RNA/DNA)
9/10
Stefanie Brady Industrial Lecture Series (1):
Engineers in Biotech
9/13 Enzymes
Michaelis-Menten Kinetics
Michaelis-Menten Rate Expression
, derivation & reduction.
9/15
Integrate ODEs Arising from Enzyme Kinetic Mechanisms
9/17 Video: The Rise of a Wonder Drug
9/20
Enzyme bioreactor (Batch, Fed-batch, CSTR)
9/22 Enzyme bioreactor (Batch, CSTR, Fed-batch)
9/24
Stefanie Brady Industrial Lecture Series (2):
Overview of Processing (Upstream, Recovery, Downstream); MFG Process; PFD's, Batch Records, cGMP ...
9/27 Enzyme Kinetics: Inhibition
Symbolic Derivation of Reaction Rate Expression with Enzyme Inhibition (Competitive, Noncompetitive, Uncompetitive)
Symbolic Derivation of Reaction Rate Expression with Substrate Inhibition
Product inhibition
9/27
pH Effect
, Temperature Effects
9/29 Enzyme Immobilization, External Mass Transfer Resistance
Surface-Immobilized Enzyme -- Effectiveness Factor & Apparent Reaction Rate
10/01 Enzyme Immobilization, External Mass Transfer Resistance
Surface-Immobilized Enzyme -- Steady-State CSTR with Product Inhibition
Surface-Immobilized Enzyme -- Batch Bioreactor
10/04 Enzyme Immobilization, Internal Mass Transfer Resistance
Demo (Catalyst Supports)
Demo (Gel Entrapment)
10/06 Enzyme Immobilization, Internal Mass Transfer Resistance
Enzyme Immobilization in Gel Beads & Strings -- Derivation & Use of Effectiveness Factor
Ditto, /w Variation
Enzyme Immobilization in a Spherical Gel-Bead -- Substrate/Product Profile with Substrate/Product Inhibition
10/08
Quiz #1
10/11
NCBI (National Center for Biotechnology Information)
, GenBank, BLAST, Human Genome Project
10/13 Central Dogma of Biology: Replication, DNA Sequencing, PCR
DNA Sequencing Gel Pattern
10/15 Central Dogma of Biology: Transcription, Promoter/Operator, Induction/Repression, Translation
Codon Translation Table
Central Dogma of Biology
(Artificial Sequence)
Genetic Codes from Internet (Human Insulin) (
insulin.seq
) (
Annotated
)
10/18 Mutation, Site-Directed Mutagenesis, Deletion/Addition of Bases
10/20 Genetic Engineering, Restriction Enzyme, Ligase, Transformation, Transfection
A List of Restriction Enzymes from Sigma
Find restriction site with
NEBcutter
from
New England BioLabs
10/22 Common Bacterial Cloning Vectors
pBR322
pUC19
,
pUC19 (alternate)
,
pUC18
,
Summary of pUC19
Cloning Example:
Cloning Luciferase into pBR322
10/25 Metabolic Pathways (Oxidation/Reduction, NAHD, ATP)
Biochemical Pathways Wall Chart
(from Sigma-Aldrich)
Biochemical Pathways Wall Chart
(from ExPASy)
KEGG Pathway Maps
10/27 Metabolic Pathways (Glycolysis & TCA), Energy Efficiency
Glycolysis
(
annotated
)
TCA Cycle
(
annotated
)
Energy Efficiency
10/29 Cell Growth Kinetics
Estimate Growth and Yield Parameters from Batch Fermentation Data
11/01
Optimial Dilution Rate for Maximum Cell Productivity
11/03 Dynamic Simulation of Bioreactor
Dynamic Simulation of Continuous Fermentor
11/03 Steady-State Stability Analysis
11/05
Stefanie Brady Industrial Lecture Series (3):
Basic Bioreactors - Parts and Operation
11/08
Quiz #2
11/09 Field Trip to
BioFactura
11/12 Field Trip to
Human Genome Sciences
11/15
Structured Model with Intracellular Substrate, Precursors, and Macromolecules
11/17
Fed-Batch Fermentor, Dynamic Simulation
11/19
Stefanie Brady Industrial Lecture Series (4):
Contamination / Viral Prevention and Safety
11/22 Dynamic Simulation (Plasmid Instability),
Two-Compartmental Model for Incomplete Mixing
Cell Recycle
11/24 Stoichiometry
Stoichiometry: Ethanol yield as a function of Respiratory Quotient (RQ)
utilize glucose to produce ethanol
Stoichiometry: Biomass yield as a function of Respiratory Quotient (RQ)
utilize ethanol to produce acetic acid
Biomass Estimation from OUR Measurement
11/26 Holiday (Thanksgiving)
11/29
Stefanie Brady Industrial Lecture Series (5):
Process Controls and Trouble Shooting
12/01 Oxygen Mass Transfer, Scale-Up
12/03
Stefanie Brady Industrial Lecture Series (6):
Bioreactor Scale-up / Computational Fluid Dynamics
12/06 Purification
Demo (Ammonium Sulfate Precipitation, Chromatography, Dialysis, Gel Permeation, etc)
12/08 Purification
12/10 Semester Review
12/18 8am-10am
Final Examination
Return to Prof. Nam Sun Wang's
Home Page
Biochemical Engineering
Forward comments to:
Nam Sun Wang
Department of Chemical & Biomolecular Engineering
University of Maryland
College Park, MD 20742-2111
301-405-1910 (voice)
301-314-9126 (FAX)
e-mail:
nsw@umd.edu
©2010 by Nam Sun Wang
