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Nam Sun Wang
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ENCH482
Biochemical Engineering
Fall 2009
Homework
Class Syllabus
Policy on Academic Integrity
8/31 Syllabus, Terminology, Chemical Engineering vs. Biochemical Engineering
9/02 Video: The Rise of a Wonder Drug
9/04 Brief History, Applications, Nomenclature, Classification, Kingdom of Protists, Virus, Bacteria, Yeasts, Procaryotes/Eucaryotes
9/07 Biochemistry (Carbohydrates, Lipids)
9/09 Biochemistry (Amino Acid, Protein)
List of Amino Acids
Zwitterion Behavior of Amino Acid
9/11 Biochemistry (RNA/DNA)
9/14
Demo (Density Gradient Centrifugation)
, Enzymes
9/16 Review of Numerical Methods
9/18 Michaelis-Menten Kinetics
Michaelis-Menten Rate Expression
, derivation & reduction.
9/21 Michaelis-Menten Kinetics
Integrate ODEs Arising from Enzyme Kinetic Mechanisms
9/23 Enzyme bioreactor (Batch, CSTR, Fed-batch)
9/25 Enzyme Kinetics: Inhibition
Symbolic Derivation of Reaction Rate Expression with Enzyme Inhibition (Competitive, Noncompetitive, Uncompetitive)
Symbolic Derivation of Reaction Rate Expression with Substrate Inhibition
Product inhibition
9/28
pH Effect
, Temperature Effects
9/30 Enzyme Immobilization, External Mass Transfer Resistance
Surface-Immobilized Enzyme -- Effectiveness Factor & Apparent Reaction Rate
10/02 Enzyme Immobilization, External Mass Transfer Resistance
Surface-Immobilized Enzyme -- Steady-State CSTR with Product Inhibition
Surface-Immobilized Enzyme -- Batch Bioreactor
10/05 Enzyme Immobilization, Internal Mass Transfer Resistance
Demo (Catalyst Supports)
Demo (Gel Entrapment)
10/07 Enzyme Immobilization, Internal Mass Transfer Resistance
Enzyme Immobilization in Gel Beads & Strings -- Derivation & Use of Effectiveness Factor
Ditto, /w Variation
Enzyme Immobilization in a Spherical Gel-Bead -- Substrate/Product Profile with Substrate/Product Inhibition
10/09
Quiz #1
10/12
NCBI (National Center for Biotechnology Information)
, GenBank, BLAST, Human Genome Project
10/14 Central Dogma of Biology: Replication, DNA Sequencing, PCR
DNA Sequencing Gel Pattern
10/16 Central Dogma of Biology: Transcription, Promoter/Operator, Induction/Repression, Translation
Codon Translation Table
Central Dogma of Biology
(Artificial Sequence)
Genetic Codes from Internet (Human Insulin) (
insulin.seq
) (
Annotated
)
10/19 Mutation, Site-Directed Mutagenesis, Deletion/Addition of Bases
10/21 Genetic Engineering, Restriction Enzyme, Ligase, Transformation, Transfection
A List of Restriction Enzymes from Sigma
Find restriction site with
NEBcutter
from
New England BioLabs
10/23 Common Bacterial Cloning Vectors
pBR322
pUC19
,
pUC19 (alternate)
,
pUC18
,
Summary of pUC19
Cloning Example:
Cloning Luciferase into pBR322
10/26 Metabolic Pathways (Oxidation/Reduction, NAHD, ATP)
Biochemical Pathways Wall Chart
(from Sigma-Aldrich)
Biochemical Pathways Wall Chart
(from ExPASy)
KEGG Pathway Maps
10/28 Metabolic Pathways (Glycolysis & TCA), Energy Efficiency
Glycolysis
(
annotated
)
TCA Cycle
(
annotated
)
Energy Efficiency
10/30 Cell Growth Kinetics
Estimate Growth and Yield Parameters from Batch Fermentation Data
11/02
Optimial Dilution Rate for Maximum Cell Productivity
11/04 Dynamic Simulation of Bioreactor
Dynamic Simulation of Continuous Fermentor
11/04 Steady-State Stability Analysis
11/06
Steady-States & Stability Analysis /w Andrew's Substrate Inhibition Model
Linearized Stability Analysis of Continuous Fermentor
11/09 Guest lecture by Ben Woodard of BioScale Up Facility (BSF)
11/11
Quiz #2
11/13 Field Trip to Human Genome Sciences
11/16
Structured Model with Intracellular Substrate, Precursors, and Macromolecules
11/18
Fed-Batch Fermentor, Dynamic Simulation of
11/20 Dynamic Simulation (Plasmid Instability),
Two-Compartmental Model for Incomplete Mixing
Cell Recycle
11/23 Stoichiometry
Stoichiometry: Ethanol yield as a function of Respiratory Quotient (RQ)
utilize glucose to produce ethanol
Stoichiometry: Biomass yield as a function of Respiratory Quotient (RQ)
utilize ethanol to produce acetic acid
Biomass Estimation from OUR Measurement
11/25 Oxygen Mass Transfer, Scale-Up
11/27 Holiday (Thanksgiving)
11/30 Instrumentation, Sensor
12/02 Sterilization, Heat Transfer,
Steam Sterilization
12/04 Purification
Demo (Ammonium Sulfate Precipitation, Chromatography, Dialysis, Gel Permeation, etc)
12/07 Purification
12/09 Purification
12/11 Semester Review
12/19 8am-10am
Final Examination
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Biochemical Engineering
Forward comments to:
Nam Sun Wang
Department of Chemical & Biomolecular Engineering
University of Maryland
College Park, MD 20742-2111
301-405-1910 (voice)
301-314-9126 (FAX)
e-mail:
nsw@umd.edu
©2009 by Nam Sun Wang
